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Foot-and-mouth disease (FMD) is an acute, severe, and highly contagious infectious disease caused by foot-and-mouth disease virus (FMDV), which seriously endangers the development of animal husbandry. The inactivated FMD vaccine is the main product for the prevention and control of FMD, which has been successfully applied to control the pandemic and outbreak of FMD. However, the inactivated FMD vaccine also has problems, such as the instability of antigen, the risk of spread of the virus due to incomplete inactivation during vaccine production, and the high cost of production. Compared with traditional microbial and animal bioreactors, production of antigens in plants through transgenic technology has some advantages including low cost, safety, convenience, and easy storage and transportation. Moreover, since antigens produced from plants can be directly used as edible vaccines, no complex processes of protein extraction and purification are required. But, there are some problems for the production of antigens in plants, which include low expression level and poor controllability. Thus, expressing the antigens of FMDV in plants may be an alternative mean for production of FMD vaccine, which has certain advantages but still need to be continuously optimized. Here we review the main strategies for expressing active proteins in plants, as well as the research progress on the expression of FMDV antigens in plants. We also discuss the current problems and challenges encountered, with the aim to facilitate related research.
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Animals , Foot-and-Mouth Disease Virus/genetics , Foot-and-Mouth Disease/prevention & control , Antigens, Viral/genetics , Viral VaccinesABSTRACT
Laparoscopic decortication is the standardized surgical treatment for simple renal cysts. With the continuous maturation of minimally invasive techniques, percutaneous nephroscopic decortication and decompression is gradually implemented, which has advantages of minimal invasiveness, fast recovery and good prognosis. Ureteroscopic plasma electrode is a modified procedure, which can achieve more exact intraoperative hemostasis and efficient cutting efficiency than traditional surgery. This article reviews the current treatments of simple renal cysts, and introduces our experience of using minimally invasive percutaneous nephroscopic plasma electrode decortication, its surgical procedures and technical points.
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Objective:To compare the effectiveness and safety of laparoscopic hepatectomy (LH) versus open hepatectomy (OH) for intrahepatic cholangiocarcinoma(ICC).Methods:PubMed, Web of Science, Embase, Cochrane Library, China Biology Medicine Disc, China National Knowledge Infrastructure, Wanfang Database and VIP Database were searched from inception of these databases to May 2022 to compare LH versus OH for ICC. The duration of operation, intraoperative bleeding, proportion of patients with intraoperative blood transfusion, number of resected lymph nodes, proportion of patients with R 0 resection margin, duration of hepatic occlusion, hospital stay, incidence of postoperative complication and postoperative mortality were compared between the two groups. Meta-analysis was carried out using the Review Manager 5.1 software, and the mean difference ( MD) or odds ratio ( OR) was used as the effect index. Results:This meta-analysis included twelve articles, all of which were retrospective cohort studies, with 3 189 patients. There were 667 patients in the LH group and 2 512 in the OH group. Meta-analysis showed that when compared to the OH group, the LH group had significantly less intraoperative bleeding ( MD=-116.06, 95% CI: -173.07--59.06, P<0.001), less proportion of patients receiving intraoperative blood transfusion ( OR=0.25, 95% CI: 0.10-0.62, P=0.003), less number of lymph nodes removed ( MD=-101.91, 95% CI: -124.78--79.03, P<0.001), less patients underwent portal occlusion ( OR=0.37, 95% CI: 0.14 - 0.99, P=0.050), shorter hospital stay ( MD=-2.43, 95% CI: -4.59--0.28, P=0.030) and less postoperative complications ( OR=0.41, 95% CI: 0.28-0.61, P<0.001). However, the proportion of patients with R 0 margin ( OR=1.49, 95% CI: 1.14-1.95, P=0.003) in the LH group was significantly higher than the OH group. There were no significant differences in operative time and postoperative mortality between the 2 groups. Conclusion:LH was more effective and safe than OH in the treatment of ICC. However, its long-term effect still needs to be verified by large randomized controlled trials.
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Background Female office workers are prone to fatigue due to work and life stress. The natural environment has a positive effect on health, but little research has focused on the effects of forest therapy on physical and mental health of female office workers. Objective To explore the effect of forest therapy on the mental health of fatigued female office workers. Methods In this study, two environments, urban and forest, were selected. A total of 12 subjects with fatigue were recruited and randomly divided into two groups, urban and forest, six subjects in each group, with mean ages of 24.7 and 28.4 years, respectively, all of whom were company employees. The Brief Mood Scale, the Chinese version of Stress Self-Rating Scale, and the Fatigue Self-Rating Scale were distributed to assess baseline psychological indexes of mood, fatigue, and stress status. Higher scores indicate higher levels of negative mood, fatigue, and stress, respectively. The forest group participated in an on-site experiment using forest therapy (forest walks and sits in a forest environment), and the urban group followed the same schedule as the forest group conducting walks and sits in an urban environment. Salivary cortisol was used as the physiological index. Baseline physiological indices were collected on the first day of the experiment, and the on-site forest therapy experiment and collecting physiological and psychological indices were conducted on the second day; physiological indices were collected in the morning before the walk and physiological and psychological indices were collected after the walk, physiological indices were collected in the afternoon before and during the sitting, and psychological indices were collected after the sitting. Subjects' mood and fatigue status were followed up one week after the activity. Mann-Whitney U test and Wilcoxon signed rank test were used to assess the changes of the indicators. Results As to the physiological indicator, the salivary cortisol concentrations before and after the morning walk in the forest group were lower than the corresponding baseline values (both P=0.043); and the decreases were 2.21 and 1.86 nmol·L−1 respectively. Differences between groups showed a trend towards a greater decrease in salivary cortisol concentrations in the forest group than in the urban group after walking and at the 15 th min of sitting (both P=0.068). Among the mood indicators, the forest group showed a greater decrease than the urban group in fatigue dimension after walking (P=0.065) and after sitting (P=0.024); the forest group showed a greater decrease than the urban group in vitality dimension after walking (P=0.054) and after sitting (P=0.045); and the forest group showed a greater decrease than the urban group in "total mood disorder" after sitting (P=0.054). Among the fatigue indicators, the forest group showed a greater decrease in "physical fatigue", "mental fatigue", "consequences of fatigue", and "general fatigue" after walking and sitting than the urban group (all P<0.05). Among the stress indicators, there were not statistically significance of "tension", "loss of control", and "total stress score" between the two groups after sitting (both P>0.05). After one week after the activity, there were not statistically significant differences of mood and fatigue between the two groups (both P>0.05). Conclusion Forest therapy has a regulating effect on symptoms of negative mood, and fatigue in fatigued female office workers, but the effects of a short-term forest therapy last for a limited duration.
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Background It has been proved by many studies that forest environment has good restorative effects. Some researchers have attempted to identify natural factors affecting restorativeness. However, current studies have not focused on the effect of seasonal variation of forest environments on its restorative strength. Objective To evaluate the restorative effect changes of two types of forest environments across different seasons. Methods The experiments were conducted in April (spring), June (summer), and October (autumn) of 2017. Forty subjects were recruited in spring，18 in summer, and 20 in autumn. In spring, the subjects were randomly assigned to a forest park in suburban area and one in semi-pristine area in Beijing where each subject only experienced one kind of forest environment. In summer and autumn, each subject experienced two kinds of forest environments. They spent the first day in the suburban area forest park, and the second day in the semi-pristine area forest park. First the subjects completed the Brief Profile of Mood States (BPOMS) and had their heart rate been monitored for 10 min before test followed by a 30 min forest experience with sedentary sitting. At the end of each experience, the subjects completed the BPOMS again, and also the Restoration Environment Scale-Chinese version. Objective physical environment indicators were monitored simultaneously during forest experience. The changes of each indicator were analyzed before and after forest experience in selected three seasons. Results In the objective physical environment indicators, the concentrations of anion in selected three seasons in the semi-pristine area forest were higher than the values in the suburban development area forest; the concentration of anion in autumn was much higher than those in the other two seasons. In the assessment of the Restorative Environment Scale, the total scores of restorativeness of the semi-pristine area forest were generally higher than the scores in the suburban development area forest, but significant differences were found only in spring and summer (P < 0.001 in spring, P=0.022 in summer, and P=0.083 in autumn). In terms of physiological indicators, the average heart rate was decreased, while short-term R-R interval standard deviation (SD1) and root mean square of difference of successive intervals (RMSSD) were increased after the designed forest experience sessions in the three seasons, and there was statistical difference between forest types. In terms of psychological indicators, a decrease in the score of total mood disorder was presented after the experience session. In spring, there was a trend that the forest park in suburban development area had a better regulation effect on total mood disorder (P=0.07). In summer and autumn, there was a trend that the semi-pristine area forest park had a better regulation effect on total mood disorder (P=0.062 in summer and P=0.001 in autumn). Conclusion Generally, forest environments can improve the psychological and physiological indicators of human, and the improvement varies with frosts types and seasons. The restorative effects of forests in semi-pristine areas is better than those in suburban development area.
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Objective To analyze the clinical characteristics and prognostic risk factors of helicobacter pylori (Hp) infected patients under 30 years old, and to provide a theoretical basis for the eradication of Hp in young Hp-positive patients under 30 years old. Methods A total of 565 patients with upper gastrointestinal symptoms admitted to our hospital from December 2017 to December 2020 were selected. All patients were Hp positive, and all patients were treated with quadruple eradication therapy of Helicobacter pylori. According to the Hp negative status after treatment, the patients were divided into good prognosis group (Hp negative, n=374) and poor prognosis group (Hp not negative, n=191). A self-made questionnaire was used to analyze the age, gender, familial history of gastrointestinal diseases, life habits (long-term smoking, and drinking), eating habits (irregular diet, and excitant food), DOB baseline value, and treatment compliance of the two groups. Logistic regression was used to analyze the factors that may affect the prognosis of HP positive patients. Results Among the 565 Hp positive patients, 347 were males and 218 were females, with an average age of (23.49±4.29) years, 393 (69.56%) aged 18-24 years old and 172 (30.44%) aged 25-29 years old. The diagnosis results of upper gastrointestinal diseases were 54 cases of gastric cancer (9.56%), 229 cases of peptic ulcer (40.53%), 174 cases of chronic gastritis (30.80%), and 108 cases of reflux esophagitis (19.12%). There were no significant differences in age, sex, family history of gastrointestinal diseases and alcoholism between the two groups (P>0.05). There were statistically significant differences between the two groups in long-term smoking, irregular diet, preference for stimulating food, treatment compliance and baseline value of DOB (P<0.05). The results of logistic regression analysis showed that long-term smoking, poor treatment compliance and high DOB value were independent risk factors affecting the prognosis of Hp positive patients (P<0.05). Conclusion Chronic gastritis and peptic ulcer are the main upper gastrointestinal diseases in Hp-positive young patients under 30 years old. Long-term smoking, poor treatment compliance and high DOB value are independent risk factors affecting the prognosis of patients. Treatment compliance of patients should be improved, and intervention should be strengthened in patients with high DOB value and smoking.
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Objective@#To study the safety and effectiveness of infra-hepatic inferior vena cava clamping combined with Pringle’s maneuver during hepatectomy.@*Methods@#The databases of PubMed, Cochrane Center, CNKI, VIP, Wan Fang were searched to extract the relevant data for the meta-analysis using the Revmanage 5.3 software.@*Results@#A total of 14 studies with 1595 patients were included in this meta-analysis. The results showed that total bleeding during the combined clamping was significantly lower than that of the Pringle’s maneuver alone (MD=-314.59, 95%CI: -362.37~-266.81, P<0.05). Bleeding during hepatectomy was significantly lower (MD=-217.79, 95% CI: -284.57~-151.00, P<0.05); so was the intraoperative blood transfusion rate (OR=0.48, 95% CI: 0.36~0.62, P<0.05). The incidence of complications was significantly lower (OR=0.74, 95% CI: 0.57~0.95, P<0.05). However, there were no significant differences in operation time, length of hospital stay, intraoperative monitoring index and postoperative liver and kidney function recovery (P>0.05).@*Conclusions@#Infra-hepatic inferior vena cava clamping combined with Pringle’s maneuver in hepatectomy was safe and reliable. Compared with the Pringle’s maneuver alone, the combined technique reduce bleeding and the incidence of complications. This technique is worth promoting in clinical practice.
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Objective To study the safety and effectiveness of infra-hepatic inferior vena cava clamping combined with Pringle' s maneuver during hepatectomy. Methods The databases of PubMed, Cochrane Center, CNKI, VIP, Wan Fang were searched to extract the relevant data for the meta-analysis using the Revmanage 5. 3 software. Results A total of 14 studies with 1595 patients were included in this meta-analysis. The results showed that total bleeding during the combined clamping was significantly lower than that of the Pringle's maneuver alone (MD= -314. 59, 95％CI:-362. 37～ -266. 81, P<0. 05). Bleeding during hepatectomy was significantly lower ( MD= -217. 79, 95％ CI: -284. 57 ～ -151. 00, P<0. 05); so was the intraoperative blood transfusion rate ( OR = 0. 48, 95％ CI: 0. 36 ～ 0. 62, P<0. 05). The incidence of complications was significantly lower (OR =0. 74, 95％ CI: 0. 57 ～0. 95, P<0. 05). However, there were no significant differences in operation time, length of hospital stay, intrao-perative monitoring index and postoperative liver and kidney function recovery ( P >0. 05 ) . Conclusions Infra-hepatic inferior vena cava clamping combined with Pringle' s maneuver in hepatectomy was safe and reliable. Compared with the Pringle' s maneuver alone, the combined technique reduce bleeding and the incidence of complications. This technique is worth promoting in clinical practice.
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Objective To investigate the MRI features and characteristics of irregular intraductal papilloma (IDP)and invasive ductal carcinoma (IDC),and to improve the diagnostic accuracy of MRI.Methods This study retrospectively included 3 3 patients with IDP and IDC confirmed by surgery and pathology,and analyzed MRI findings including lesion size,boundary,internal components,plain signal intensity,enhancement mode,ADC value and TIC curve χ.2 test,t test and rank-sum test were performed.Results Compared with the two groups,lesion boundary (P＜0.001),size (P＜0.001 ),ADC value (P＜0.001 ),enhancement mode (P＝0.001 ),TIC curve peak time (P＜0.001),slope (P＜0.001)and peak enhancement rate (P＜0.001)were statistically significant (P＜0.05);lesion (P＝0.159), internal components (P＝0.778),T2 WI signal (P＝0.438)and curve type (P＝0.406)were no statistically difference.Conclusion The irregular mass type IDP and IDC have similar MRI findings,the edge of the lesion,the ADC value,the enhancement mode,and the peak time,slope and peak enhancement rate of the curve are important for the identification of the two diseases.
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Objective To investigate the opportunity and skill of surgery for pancreatic sinistral portal hypertension.Methods Clinical data were retrospectively analyzed on 15 cases of pancreatic sinistral portal hypertension admired from Dec 2015 to Dec 2017.Results All fiften cases underwent surgical treatment,among them three cases were initially treated conservatively in the early stage and treated surgically for gastrointestinal bleeding,12 cases with definite pancreatic disease and pancreatic sinistral portal hypertension treated in the first stage.Three patients underwent second surgery for recurrent gastrointestinal bleeding.The patients were followed up for 6 to 18 months with symptoms significantly impioved without deaths.Conclusions Splenectomy combined with esophagogastric devascularization is the basic surgical treatment for pancreatic sinistral portal hypertension.
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Objective To study the distribution and the characteristic of elbow fractures in children according to the age,sex,year,season and anatomical location.Methods The data of pediatric elbow fractures which were treated either in outpatient department or hospitalized in the First Affiliated Hospital of Guangxi Medical University between January 2011 and December 2016 were collected.The distribution and characteristics of the fractures were analyzed by using SPSS 18.0 software and Excel 2010.Results A total of 613 patients with elbow fractures (628 different anatomical sites) were registered.The incidence seemed to increase by each year.Among them,414 cases were male and 199 cases were female (male/female ratio 2.08 ∶ 1.00).There were 3 distinct peaks which were respectively at the ages of 1,3 and 5.The fractures happened frequently from April to October.The most common type was supracondylar fracture of humerus(71.29％,437/613 cases),followed by lateral condylar fracture of humerus(14.68％,90/613 cases),olecranon fracture(3.26％,20/613 cases) and radial neck fracture (2.28％,14/613 cases).The most common cause of injury was falling on the same level (67.54％,414/613 cases),followed by dropping from heights (16.64％,102/613 cases),then sports injury (7.83％,48/613 cases),traffic accidents (5.55％,34/613 cases) as well as some intentional injuries (0.65％,4/613 cases).Conclusions The highest incidence of elbow fracture was found in children around 1 year,and 3-6 years.Boys are more affected than girls,probably attributed to their active nature.Fractures are more frequent in summer in which supracondylar fracture of humerus is the most common type.Most fractures occur after falling on the same level,indicating how easy it is to get injured in children.So careful examination is necessary to all the pediatric cases who came with a history of fall.Attention should be paid to intentional injuries especially so as to rule out if the cases belong to brutal abuse.
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Objective To improve the diagnostic accuracy of nonpalpable calcified breast lesions by establishing a Logistic multivariate prediction model to assess the probability of benign/malignant breast lesions.The proposed model is based on the clinical and BI-RADS-X-ray imaging features of patients with nonpalpable calcified breast lesions.Methods A total of 147 nonpalpable calcified breast lesions were analyzed retrospectively.Firstly, based on the personal experience,the X-ray imaging data of lesions were analyzed to obtain the BI-RADS categorization, and the ROC curve was plotted by comparison with pathology.Then the univariate and multivariate analysis was performed on the clinical and X-ray imaging features of pathology to select the independent factors related to benign/malignant features.Further,a Logistic regression model was built,the suitable cut-off point was determined, and the ROC curve was obtained.Finally,the comparisons of the diagnostic accuracy of breast lesions were made between the method using the BI-RADS categorization and the method using the Logistic regression model.Results The AUC of the BI-RADS method was 0.867 9.The univariate analysis showed that there exist statistical differences among clinical features of patients(age,location,and quadrant),as well as the BI-RADS-X-ray imaging features (distribution,morphological and gland density).Also,by using the multivariate Logistic regression equation,the statistical differences among age,quadrant and morphological difference can be observed.The AUC using the built Logistic regression model was 0.906 3.Conclusion The diagnostic accuracy of breast lesions using the Logistic model is higher than that using the BI-RADS categorization method.Therefore, the proposed model is valuable for obtaining accurate diagnosis of breast lesions.
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The evaluation tools were developed based on the theory of validity,the development process and results of the Manual of Home-Based Rehabilitation Mode for Stroke Patients were evaluated by a panel of experts,and the opinions and suggestions for improvement were collected from the evaluation experts.The results showed that the average score for development process and results of the manual were ＞4 points in structural validity,content validity and surface validity,indicating that the manual was of high quality,and the development of the manual was scientific and the results were practical.The manual may provide the guidance for the home-based rehabilitation in community.At the same time,this study has enriched the reviewing tools and evaluation method of manuals,and provided reference for the peers.
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Sixteen experts were invited and two-round Delphi expert consultation of admission and discharge criteria for stroke patients in home-based rehabilitation were conducted from April to October 2015.Results showed that the enthusiasm and authority degree of experts in two rounds consultation were favorable.The modified admission criteria included 8 inclusive conditions and 6 exclusive conditions.Among 8 inclusive conditions,home-based rehabilitation intervention time and NIHSS score were the necessary conditions for admission,in addition to 6 exclusion conditions.With the necessary conditions,if patients had disturbances of one or more following conditions:motor function,tactile and proprioceptive function,communication function,cognitive function,quality of life and other symptoms,they were eligible to be admitted in home-based rehabilitation in community.The discharged criteria contained two aspects:1.the conditions in admitted criteria reaching the plateau;2.the occurrence of unexpected situations,including disease worsening,deep vein thrombosis and stroke recurrence.The admission and discharged criteria developed in this study has high credibility and can be recommended for practical application in other communities in home-based rehabilitation of stroke patients,which also can be used for implementation of level-classified rehabilitation for stroke patients.
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The community rehabilitation model for stroke patients was developed based on the experiences of Quyang Community Health Service Center in Shanghai under the guidance of Lean Six Sigma.The validity of the process and results were evaluated by a expert group according to validity theory.The developed Manual of Home-based Rehabilitation mode for Stroke Patients in Community had good validity,which may provide guidance for stroke patients rehabilitation in the community,and also provide reference for clinical application.
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As a new service form,community medical service provides primary health care for residents and it is operated with reasonable medical expenses.The function and service range of community medical service have reached consensus in medical industry,but the key issues of how to gain the market by competitive products and reasonable price have not been solved.This article analyzes the position of community medical service in healthcare system to clarify its unique attributes.The article further discusses the contents and pricing characteristics of community medical service,and the main influencing factors.The article introduces the issues dealing with community medical service product development,including the procedure of development,key points at different stages of development and the related research methods for the development of new service products.
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The current workflow in home-based rehabilitation for stroke patients were modified through focus group discussion,brainstorming and literature review.The mapping of three-tier rehabilitation network,the defined function of home-based rehabilitation and its integration with other rehabilitation services were made by literature review.The improved version of home-based rehabilitation flow chart specified the contents in the guideline Three-tier rehabilitation network of stroke rehabilitation in China.The chart provides a reference for the implementation of home-based rehabilitation in community health service centers with the family doctor system.
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Based on the experiences in Quyang Community Health Service Center,and through focus group discussions and literature research,the home-based rehabilitation mode for stroke patients in community was developed.With the principles of safety and effectiveness,rehabilitation physicians and family physicians are responsible for rehabilitation program,while rehabilitation therapists and community nurses implement the rehabilitation program.The rehabilitation services are provided for patients and their families at home with the community service resources,including counseling services,rehabilitation aids and other cares according to their needs and demands.The definition and contents of the mode provide a basis for further clarification and operation of home-based rehabilitation for stroke patients in community.
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Objective To assess the value of diffusion weighted imaging (DWI) in monitoring therapy effect of neoadjuvant chemotherapy (NAC) in patients with locally advanced breast cancer.Methods The prospective study included 26 lesions in 24 patients with LABC undergoing NAC.MRI was conducted before chemotherapy,after the first cycle of chemotherapyand after all cycles,including conventional MRI and DWI.All patients were confirmed by pre-treatment fine needle aspiration biopsy (FNAB),and postoperative histopathological prout.All lesions were divided into two groups of pathological complete response (pCR) and non-pCR.We evaluated ADC value and its changes in pathological complete response difference between the groups and complete remission in each testing point.Results Before chemotherapy and after the first cycle of chemotherapy,the ADCmean andADCmin had no statistically significant difference hetween pathologic complete response group and non-complete remission.After the whole chemotherapy,both of them had statistically significant difference;pathological complete response (pCR) and non-pCR early ADCmin value rate were (16.78 ± 22.70) ％ and (12.37 ± 10.09) ％respectively.No statistically significant difference was seen (P =0.53).Preoperative ADCmin value rates were (91.41 ± 45.83) ％ and (29.74 ± 22.96) ％ respectively,which were statistically significant (P =0.00) Conclusion ADC which is measured from DWI can be used to assess early response to NAC in breast cancer lesion,but cannat predict pCR or non-pCR for all cycles of chemotherapy on the first cycle.
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BACKGROUND: Low intensity pulsed ultrasound is safe, non-invasive, and has strong penetration. In recent years, more and more studies have indicated that low intensity pulsed ultrasound has important role in promoting the repair of damaged articular cartilage. OBJECTIVE: To investigate the effect of low intensity pulsed ultrasound on the expression of type II col agen and matrix metal oproteinase 13 in articular cartilage of rabbits of knee arthritis. METHODS: A total of 20 New Zealand white rabbits were included in this study to establish knee arthritis model. Al models were randomly divided into control group and experimental group, 10 rats in each group. In the experimental group, the right knee joint received low intensity pulsed ultrasound treatment with an ultrasonic fracture healing instrument. In the control group, right knee joint received false low intensity pulsed ultrasound therapy. At 8 weeks after intervention, cartilage tissue was col ected from medial end of tibia and medial end of femur. After conventional treatment, toluidine blue staining was performed. Pathological observation was conducted under a microscope. Quantitative real-time polymerase chain reaction was used to detect matrix metal oproteinase 13 and type II col agen expression in chondrocytes. The general situation of the experimental animals was observed. The results of articular cartilage and the quantitative real-time polymerase chain reaction results of matrix metal oproteinase-13 and type II col agen were observed under a microscope. RESULTS AND CONCLUSION: (1) Toluidine blue staining and pathological observation: 8 weeks after treatment, obvious fissure appeared in articular cartilages, and extending from the surface to the deep layer in the control group, and there was a lack of staining. In the experimental group, cracks formed on articular cartilage surface, and there was a moderate staining missing. (2) Quantitative real-time polymerase chain reaction: at 5 and 10 days after culture, significant differences in gene expression of matrix metal oproteinase-13 and type II col agen were detected at various time points in the experimental and control groups (P < 0.05). (3) The results showed that the low intensity pulsed ultrasound could affect the gene expression of matrix metal oproteinase-13 and type II col agen in the rabbit model of knee arthritis.